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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and <t>Lrp5</t> −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.
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Mice body weight and mice adipose tissue weight. (A) Wt and Lrp5 −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Mice body weight and mice adipose tissue weight. (A) Wt and Lrp5 −/− mice weight gain after 8 weeks of NC diets. (B) VAT, (C) SCAT, (D) EAT, and (E) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (F) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in NC mice. (G) Wt and Lrp5 −/− mice weight gain after 8 weeks of HC diet feeding. (H) VAT, (I) SCAT, (J) EAT, and (K) BAT weights from Wt and Lrp5 −/− mice at sacrifice. (L) Wt and Lrp5 −/− mice total adipose tissue weight against mice total body weight in HC mice. (M) Significance between NC vs. HC Wt mice and NC vs. HC Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques:

Low‐density lipoprotein receptors gene expression in mice and humans. (A) Lrp5 gene expression in adipose tissue of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis for (B) Lrp5 levels in VAT vs. VAT weight in Wt mice and (C) Lrp5 expression in SCAT vs. SCAT weights in Wt mice. (D) Lrp1 gene expression in adipose tissue of NC/HC Wt and Lrp5 −/− mice. Regression analysis for (E) Lrp1 levels in VAT vs. VAT weights in Wt and Lrp5 −/− mice and (F) Lrp1 levels in SCAT vs. SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. (G) Lrp5 and (H) Lrp1 gene expression in adipose tissue of lean and obese patients. n = 3–5 subjects/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Low‐density lipoprotein receptors gene expression in mice and humans. (A) Lrp5 gene expression in adipose tissue of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis for (B) Lrp5 levels in VAT vs. VAT weight in Wt mice and (C) Lrp5 expression in SCAT vs. SCAT weights in Wt mice. (D) Lrp1 gene expression in adipose tissue of NC/HC Wt and Lrp5 −/− mice. Regression analysis for (E) Lrp1 levels in VAT vs. VAT weights in Wt and Lrp5 −/− mice and (F) Lrp1 levels in SCAT vs. SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. (G) Lrp5 and (H) Lrp1 gene expression in adipose tissue of lean and obese patients. n = 3–5 subjects/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques: Gene Expression, Expressing

Adipocyte markers expression in mice. (A) Fabp4 , (B) Lpl , and (C) Cd36 expression levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Fabp4 , (E) Lpl , and (F) Cd36 gene expression in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Adipocyte markers expression in mice. (A) Fabp4 , (B) Lpl , and (C) Cd36 expression levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Fabp4 , (E) Lpl , and (F) Cd36 gene expression in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques: Expressing, Gene Expression

Macrophage markers in mice. (A) Cd68 , (B) Cd11b , and (C) F4/80 genetic levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Cd68 , (E) Cd11b , and (F) F4/80 levels in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 − / − mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Macrophage markers in mice. (A) Cd68 , (B) Cd11b , and (C) F4/80 genetic levels in adipose tissues of NC/HC Wt and Lrp5 −/− mice. Regression analysis of (D) Cd68 , (E) Cd11b , and (F) F4/80 levels in VAT and SCAT vs. VAT and SCAT weights from Wt and Lrp5 − / − mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques:

Pro‐inflammatory markers in mice adipose tissues. (A) Cd80 , (C) Nos2 , (E) Pcsk9 , and (G) Ccr7 gene expression in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (B) Cd80 , (D) Nos2 , (F) Pcsk9 , and (H) Ccr7 gene expression in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 − / − mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Pro‐inflammatory markers in mice adipose tissues. (A) Cd80 , (C) Nos2 , (E) Pcsk9 , and (G) Ccr7 gene expression in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (B) Cd80 , (D) Nos2 , (F) Pcsk9 , and (H) Ccr7 gene expression in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 − / − mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques: Gene Expression

Anti‐inflammatory markers in mice adipose tissues. (A) Cd163 , (B) Cd206 , and (C) Arg1 gene levels in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (D) Cd163 , (E) Cd206 , and (F) Arg1 levels in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Reduced Growth and Inflammation in Lrp5 −/− Mice Adipose Tissue

doi: 10.1111/jcmm.70670

Figure Lengend Snippet: Anti‐inflammatory markers in mice adipose tissues. (A) Cd163 , (B) Cd206 , and (C) Arg1 gene levels in adipose tissues of Wt and Lrp5 −/− mice fed NC or HC diets. Regression analysis of (D) Cd163 , (E) Cd206 , and (F) Arg1 levels in VAT and SCAT vs. VAT and SCAT weights in Wt and Lrp5 −/− mice. n = 8–12 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The resulting cDNA samples were amplified in a RT‐qPCR thermal cycler (Applied Biosystems) using the following probes from ThermoFisher: Lrp5 (Mm00493187_m1 / Hs00182031_m1), Lrp1 (Mm00464608_m1 / Hs00233856_m1), Fabp4 (Mm00445878_m1), Lpl (Mm00434764_m1), CD36 (Mm00432403_m1), Cd68 (Mm03047343_m1), Cd11b (Mm00434455_m1), F4/80 (Mm00802529_m1), Nos2 (Mm00440502_m1), Cd80 (Mm00711660_m1), Ccr7 (Mm00432608_m1), Pcsk9 (Mm01263610_m1), Arg1 (Mm00475988_m1), Cd163 (Mm00474091_m1) and Cd206 (Mm01329359_m1).

Techniques: